PCR Troubleshooting?
It's easy with the S-solution of Bio&SELL - the results of PCR templates with high GC content can be significantly improved by the addition of the S-solution. Even with unwanted by-products in the PCR reaction, the S-solution contributes to background minimization.
The separately packaged MgCl2 solution allows the variation of the Mg 2+ ion concentration in your PCR reaction, thereby providing for further optimization of the specific PCR reactions.
For educational purposes, you can impressively demonstrate to your students the Mg 2+ dependence of enzyme activity of DNA polymerase.
Application & Quality Control
Primer extension reactions: the enzyme is free of nicking and primers activities as well as of exonucleases and non-specific endonucleases. SDS / PAGE: 95 kD band, purity> k98%. Activity and stability were tested by PCR. The error rate per cycle per Nukeotid is 8.3x10-5 1.2x104 accuracy. The half-life at 95 °C is 90 min.
Storage and dilution buffer
50% glycerol (v / v), 20 mM Tris HCl (pH 8.7 at 25 °C), 100 mM KCl, 0.1 mM EDTA and stabilizers.
Unit definition
One unit is the amount of enzyme that is needed to convert 10 nmol of dNTP in 30 min at 74 ° C in an acid-insoluble form.
