RT-qPCR Kits for Direct RNA Analysis
In reverse transcriptase-polymerase chain reaction (RT-PCR), RNA is enzymatically transcribed into complementary DNA (cDNA) and then amplified. The enzyme that transcribes RNA into cDNA is called reverse transcriptase. The cDNA is then amplified using a polymerase. In quantitative RT-PCR (RT-qPCR), DNA amplification can be detected in real time using DNA-intercalating dyes or fluorescent probes. This allows for quantitative analysis. In one-step RT-qPCR, reverse transcription and quantitative PCR take place in the same (single) reaction vessel. This is particularly convenient when samples need to be analyzed with as few steps as possible.
The detection method is the key factor in selecting the appropriate RT-qPCR mix: SYBR® Green is used for dye-based assays, while in probe-based assays, a labeled, sequence-specific probe provides the fluorescence signal. Also, make sure that the ROX reference dye is compatible with the settings and requirements of your qPCR instrument. Find the right RT-qPCR kit for your application here!
RT-qPCR Kits from Bio&SELL:
RT-qPCR SYBR® Green Rox - One-Step Mix for dye-based RT-qPCR assays with SYBR® Green.
RT-qPCR Probe Rox - One-Step Mix for probe-based RT-qPCR assays.
FAQs
What is a One-Step RT-qPCR Kit?
In one-step RT-qPCR, reverse transcription (the conversion of RNA to cDNA) and quantitative PCR are performed in a single reaction.
When should I choose a SYBR® Green Mix?
A SYBR® Green Mix is suitable for RT-qPCR assays in which fluorescence is detected via a DNA-binding dye. Please check in advance whether this method is suitable for your experimental setup.
When is a Probe Mix the right choice?
A Probe Mix is intended for probe-based assays in which a sequence-specific probe is used for signal detection. Please verify in advance whether this method is suitable for your experimental setup.
What does ROX mean in an RT-qPCR mix?
ROX is a reference dye used for signal normalization in certain qPCR systems. Before making your selection, check whether your instrument requires ROX and what setting is intended for it.
