dNTPs

We offer you high-purity (HPLC-purified) dNTPs (dATP, dGTP, dTTP/dUTP, dCTP) both as a set (4 dNTPs in separate tubes) and as a premixed mix (all 4 dNTPs in one tube):

dNTP Set - flexible and individual
dNTP Mix - fast and convenient

When working with a dNTP set, four individual reaction tubes are obtained, each containing only one nucleotide - i.e. either dATP, dGTP, dTTP or dCTP. All four nucleotides must then be added individually for the PCR reaction. This is useful if, for example, you want to use a single nucleotide with a special label or if you want to set different concentrations for experimental reasons.

However, the most common PCR reactions require the same concentration of all four nucleotides. Here it is advisable to work with a dNTP mix. In a dNTP mix, all four nucleotides are contained in the same concentration in one reaction vessel: dATP, dGTP, dTTP and dCTP. The dNTP mix from Bio&SELL contains the nucleotides in a concentration of 20 mM each. Only one pipetting step is necessary to add all four nucleotides.

Working with a dNTP mix is convenient, saves time and material, minimizes pipetting inaccuracies and reduces the risk of contamination.

What are dNTPs? - The abbreviation dNTP stands for deoxy nucleoside triphosphate.
To synthesize the new DNA strand, DNA polymerase requires all four bases as building blocks in the form of deoxynucleoside triphosphates. The four bases are A (adenine), G (guanine), C (cytosine) and T (thymine). If the base is linked to a sugar via an N-glycosidic bond, it is referred to as a nucleoside. In DNA (= deoxyribonucleic acid), the sugar building block is deoxyribose. The four nucleosides are accordingly called deoxyadenosine, deoxyguanosine, deoxythymidine and deoxycytidine.

DNA polymerase catalyzes the incorporation of a nucleoside triphosphate at the 3`-OH end of the newly formed DNA strand, releasing pyrophosphate. The phosphate ester of a nucleoside is called a nucleotide. The OH group on the C-5 of the ribose is involved in the formation of the phosphate ester, hence the exact name for dATP, for example: deoxyadenosine 5'-triphosphate.

In every PCR reaction carried out in vitro, all four DNA building blocks must be added in the form of deoxynucleoside triphosphates (dNTPs). The nucleotides must be added in accordance with the specified optimum concentration for the DNA polymerase used in the experiment. The Bio&SELL Taq polymerase works optimally at a dNTP concentration of 200 µM, i.e. a final concentration of 200 µM must be achieved for each nucleotide in the finished PCR preparation.