Freeze Protect Medium
Our Freeze Protect media are ready-to-use freezing media that have been optimized for the cryopreservation of animal and human cells (adherent and suspension cells). The special formulation of the media guarantees very high cell viability after the thawing process.
Freeze Protect I contains various salts, sugars, DMSO and other antifreeze agents. Due to its serum-free formulation, it is particularly suitable for the preservation of serum-free cultured cells.
Freeze Protect II is a chemically defined freezing medium. It also contains various salts, sugars, DMSO and other antifreeze agents. Due to its serum-free & protein-free formulation, it is particularly suitable for the preservation of serum-free cultured cells. The risk of contamination with viruses, BSE or immunoreactive proteins is excluded with Freeze Protect II, as it does not contain any human or animal components.
Freeze Protect IV contains various salts, sugar, DMSO and other antifreeze agents and with FBS.
Article list
| Products | Article number | Quantity | Shop |
|---|---|---|---|
|
Freeze Protect I Medium
(serum-free, 10 % dmso)
|
BS33.100.050
|
50 ml
| |
|
BS33.100.100
|
100 ml
| ||
|
BS33.100.500
|
500 ml
| ||
|
Freeze Protect II Medium
(serum-free, protein-free, 10 % dmso)
|
BS33.200.050
|
50 ml
| |
|
BS33.200.100
|
100 ml
| ||
|
BS33.200.500
|
500 ml
| ||
|
Freeze Protect IV Medium
(with FBS, with 10 % dmso)
|
BS33.400.050
|
50 ml
|
Good to know
In order to create in vivo conditions for cell growth in the cell culture, serum is always added to the medium. Serum serves the cells as a source of growth factors, lipids, hormones and minerals. High-quality sera are usually expensive and the composition is not precisely defined. For this reason, serum-free media are produced. These media contain additives that enable cell cultivation without the addition of serum.
Serum-free media are generally divided into:
- Non-defined serum-free media (can have undefined plant and animal components)
- Chemically-defined serum-free media (only with components of known structure and weight)
- Protein-free media (absolutely free of proteins; usually with hydrolysates)
- Xeno-free media (free of proteins of non-human origin)
The use of serum-free media offers more consistency and reproducibility in cell culture. This makes cell cultivation more controllable and minimizes the risk of contamination. It also reduces costs by eliminating serum and simplifies downstream protein purification (protein yield is often higher).