qPCR Mastermix Probe Rox is optimized for probe-based real-time PCR and contains all components necessary for quantitative PCR except template DNA, primer and probe. qPCR Mastermix Probe Rox is 5x concentrated and contains optimized components such as our hot-start Taq DNA polymerase and a specific buffer system that enables the detection of low copy number targets.
This kit contains the reference dye Rox. Rox is a passive, internal reference dye used to normalize the fluorescence reporter signal during qPCR.