Our BioColl® separation solution contains neutral, highly cross-linked polysucrose with an average molecular weight of 400,000 Da. This hydrophilic polymer can be used to produce aqueous solutions with different densities (up to about 1.2 g/ml), which are used to isolate cells or subcellular particles. The appropriate density of the BioColl® separation solution is selected depending on the particle type. A density of 1.068 g/ml is ideal for the isolation of monocytes.
For optimum pH and osmolality values, the BioColl® separation solution should be adjusted with an acid (preferably amidotrizoic acid (ATS)) and sodium hydroxide solution.
Properties
Density: 1.068 g/ml For the isolation of human monocytes Centrifugation medium made from highly cross-linked polysucrose
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BioColl® (monocytes)
Separating solution - density 1.068 g/ml
BS.L6813
100 ml
BS.L6815
500 ml
Good to know
Cells (e.g. PBMCs) are isolated by so-called density gradient centrifugation. This physical process is based on the separation of macromolecules according to their sedimentation properties (size, weight) in a density gradient. The isolation of cells or subcellular particles is often the first step in diagnostic investigations, gene expression studies or the production of antibodies.
Which density of BioColl® separation solution do I need for which human cell fraction?
Platelet density 1.040 - 1.060 g/ml Monocyte density 1.059 - 1.068 g/ml Lymphocyte density 1.066 - 1.077 g/ml Basophil density 1.075 - 1.081 g/ml Neutrophil density 1.080 - 1.099 g/ml Eosinophil density 1.088 - 1.096 g/ml Erythrocyte density 1.090 - 1.110 g/ml
BioColl® is a registered trademark of Bio&SELL GmbH.