BioColl® (for Monocytes)
Our BioColl® separation solution contains neutral, highly cross-linked polysucrose with an average molecular weight of 400,000 Da. This hydrophilic polymer can be used to produce aqueous solutions with different densities (up to about 1.2 g/ml), which are used to isolate cells or subcellular particles. The appropriate density of the BioColl® separation solution is selected depending on the particle type. A density of 1.068 g/ml is ideal for the isolation of human monocytes. For optimum pH and osmolality values, the BioColl® separation solution should be adjusted with an acid (preferably amidotrizoic acid (ATS)) and sodium hydroxide solution.
In order to specifically separate monocytes from other mononuclear blood cells (such as lymphocytes), a double polysucrose gradient is required. For this purpose, BioColl® for monocytes (BS.L6815) is layered over BioColl® for lymphocytes (BS.L6115). Due to their higher density, lymphocytes and thrombocytes pass through the BioColl® layer for monocytes (density 1.068 g/ml), but not the BioColl® layer for lymphocytes (density 1.077 g/ml). After centrifugation, they are located between the two BioColl® layers. Monocytes do not penetrate the BioColl® layer for monocytes (density 1.068 g/ml) due to their lower density. After centrifugation, they are located between the BioColl® for monocytes (density 1.068 g/ml) and the plasma layer. The cells can be easily harvested by careful pipetting.
Properties
Density: 1.068 g/ml
For the isolation of human monocytes
Centrifugation medium made from highly cross-linked polysucrose
Good to know
Cells (e.g. PBMCs) are isolated by so-called density gradient centrifugation. This physical process is based on the separation of macromolecules according to their sedimentation properties (size, weight) in a density gradient. The isolation of cells or subcellular particles is often the first step in diagnostic investigations, gene expression studies or the production of antibodies.
What are the densities of my cells/cell particles?
| Thrombocytes | Density 1.040 - 1.060 g/ml |
| Monocytes | Density 1.059 - 1.068 g/ml |
| Lymphocytes | Density 1.066 - 1.077 g/ml |
| Basophiles | Density 1.075 - 1.081 g/ml |
| Neutrophiles | Density 1.080 - 1.099 g/ml |
| Eosinophiles | Density 1.088 - 1.096 g/ml |
| Erythroctyes | Density 1.090 - 1.110 g/ml |
Which BioColl® separation solution do I need for my cells?
| Cell type | BioColl® Separation Solution | Article Number |
| Human lymphocytes | Density 1.077 g/ml | BS.L6115 |
| Human monocytes | Density 1.068 g/ml + 1.077 g/ml | BS.L6815 + BS.L6115 |
| Human granulocytes | Density 1.077 + 1.1 g/ml | BS.L6115 + BS.L6155 |
| Murine lymphocytes | Density 1.086 g/ml | BS.L6121 |