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FBS Tetracycline-negative

Our FBS is free of tetracyclines. We control the absence of tetracyclines using the TET-off system (reporter gene: luciferase). Our product is therefore suitable for transfections, expression studies or TET-on/TET-off regulated gene expression.

Properties

No tetracyclines - Suitable for transfections or expression studies
High safety - Virus/mycoplasma tested

Article list

ProductsArticle numberQuantityShop
FBS Tetracycline-negative
FBS.TZN.0100
100 ml
FBS.TZN.0500
500 ml

Good to know

Tetracyclines are a group of antibiotics that bind and modify the 30s subunit of bacterial ribosomes. This disrupts bacterial protein synthesis and tetracyclines have a bacteriostatic effect. Tetracyclines are frequently used in animal husbandry to combat bacterial infections. In cell culture, however, they can be disruptive as they interact with pharmaceuticals and anticoagulants or form complexes with polyvalent metal ions such as Mg2+, Fe2+ and Ca2+.

We control the absence of tetracyclines in our product using the TET-off system. This system is based on the tetracycline-dependent regulation of gene expression. To initiate transcription, a tetracycline-regulated transcription factor (tTA) generally binds to the DNA sequence of its tTA-specific promoter, Ptet. However, tetracyclines interfere with the DNA binding of tTA transcription factors and thus prevent transcription of the reporter gene (luciferase).

In order to create in vivo conditions for in vitro cell culture, serum is often added to the cell culture medium. Serum serves the cells as a source of growth factors, lipids, hormones and minerals. Fetal bovine serum (FBS) is particularly rich in growth factors and contains few antibodies that could interfere with cell cultivation. This unique composition makes FBS one of the most important supplements for cell proliferation in in vitro cell culture.

Application

  • TET-on / TET-off – regulated gene expression studies
  • Transfections
  • Expression studies

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