Human serum off-the-clot

Human off-the-clot serum is derived from human whole blood and allowed to clot naturally, which is why it contains no anticoagulants. Clotting occurs at room temperature. The blood clot is then removed, and the off-the-clot serum is purified using depth and membrane filters. The off-the-clot serum contains many growth factors, vitamins, nutrients, trace elements, and transport factors.

Our off-the-clot human serum is derived from plasma or whole blood donations from exclusively healthy donors at certified institutions in Europe or the United States. Every single donation is screened for viral infections, verified, and documented. This ensures maximum safety and transparency for you.

• Without anticoagulants - Naturally coagulated
• No animal components - Produced in vivo conditions for human in vitro applications
• High safety - Every whole blood or plasma donation is intensively tested for infections
  
  

Human whole blood consists of 55% plasma and 45% solid blood components (red blood cells, white blood cells and platelets). In order to isolate plasma, anticoagulants are added to prevent the whole blood from clotting. Solid and cellular components are separated by centrifugation. The plasma then contains suspended blood cells, nutrients, hormones, minerals and proteins such as coagulation factors. Human serum corresponds to plasma fluid, without coagulation factors and cellular components. It consists of nutrients, minerals, hormones, antigens, antibodies and other proteins. To obtain serum, whole blood is either naturally clotted or isolated plasma is mixed with calcium chloride. Cellular and solid components are removed by centrifugation.

In order to create in vivo conditions for in vitro cell culture, serum is often added to the cell culture medium. Due to its unique composition, fetal bovine serum (FBS) is one of the most important supplements for cell proliferation in in vitro cell culture. However, human serum may be more suitable than FBS for culturing human cells, as both the serum and the cells to be cultured belong to the same species. If you want to convert your cells from FBS to human serum, they should be adapted to the new serum step by step. To do this, first cultivate the cells for 4-6 days with a 10% serum mixture consisting of 5% FBS and 5% human serum. Then you can finally switch the cell cultivation to human serum.

Which human sera we offer:

Human serum - Coagulated with CaCl₂
Human serum is the liquid obtained after coagulation factors have been removed from human blood plasma. Calcium chloride is added to the human plasma to induce coagulation. Cellular and solid components of the plasma are removed by centrifugation. The human serum is then washed, concentrated, and filtered.

Human AB serum (Male) - Guaranteed free of anti-A and anti-B antibodies
Our human AB serum contains only plasma from male donors with blood type AB and is free of anti-A and anti-B antibodies. For this reason, it does not react with other blood types, and unwanted immune reactions are minimized. Human AB serum is particularly recommended for immunological and hematopoietic studies.

Human off-the-clot serum - Free of anticoagulants, naturally clotted
Human off-the-clot serum is derived from human whole blood and collected without anticoagulants. Clotting occurs naturally at room temperature. The blood clot is then removed, and the serum is purified using depth and membrane filters. The serum contains many growth factors, vitamins, nutrients, trace elements, and transport factors.

Human serum AB male off-the-clot - Guaranteed free of anti-A and anti-B antibodies, naturally clotted
This human off-the-clot serum is collected exclusively from male donors with blood type AB and is free of anti-A and anti-B antibodies. The off-the-clot AB serum is derived from whole blood and collected without anticoagulants. Clotting occurs naturally at room temperature. The serum contains many growth factors, vitamins, nutrients, trace elements, and transport factors.